Chromatin Live Imaging with Genome Editing Techniques: Switching from Scissors to a Lamp
نویسندگان
چکیده
منابع مشابه
CRISPR/CAS9 Technology: A Revolutionary Molecular Scissors for Genome Editing and Genetic Research
Targeted gene modifications to animals’ especially mammalian genomes are central to understand the role of the gene in development, physiology, and different disease conditions. Functional elucidations of a gene or genetic variants require precise editing technologies. In conventional gene targeting methods, mutations of the gene or gene has been introduced into the genome by homologous recombi...
متن کامل[Scientific review on novel genome editing techniques].
背 景 次世代遺伝子組換え技術とは,欧州のJoint Research Center (JRC)が報告して以来novel plant breeding techniques (NPBTまたはNBTと略される)と呼ばれて いる新規の植物育種方法である.痕跡が残らない遺伝子組 換え法としてすでに新聞報道もされている.これらの技術 の一部は植物だけでなく動物にも用いられていることか ら,NPBTという名称は正確ではない.そのため,本総説 では次世代遺伝子組換え技術(以下,次世代組換え技術と する). 従来の遺伝子組換え作物は,優れた形質を発現させるた めに外来遺伝子を異なる生物種から分離してベクターへ組 込み後,細胞へ遺伝子導入(transfection)することによ り作出されてきた.このとき,ゲノム上にランダムに挿入 されたものの中から,望む形質のみを示すものを選抜す る.こうして得ら...
متن کاملProbing the impact of chromatin conformation on genome editing tools
Transcription activator-like effector nucleases (TALENs) and RNA-guided nucleases derived from clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 systems have become ubiquitous genome editing tools. Despite this, the impact that distinct high-order chromatin conformations have on these sequence-specific designer nucleases is, presently, ill-defined. The same applies to th...
متن کاملGenome Editing With Targeted Deaminases
Luhan Yang, Adrian W. Briggs*, Wei Leong Chew*, Prashant Mali, Marc Guell, John Aach, Daniel Bryan Goodman, David Cox, Yinan Kan, Emal Lesha Venkataramanan Soundararajan, Feng Zhang, George Church 1 Department of Genetics, Harvard Medical School, Boston, MA 2 Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 3 Harvard-MIT Division of Health Science and Technology...
متن کاملIs Genome Editing Techniques the New Challenge for Plant Breeding?
Plant breeding is the “art and science” of the genetic improvement of crops to produce new varieties that had a large impact on crop yield and food production. Genetic variation is the engine that propels breeding to meet future challenges and this variation is due to intraand inter-specific recombination. The recombinant DNA technology marked the beginning of a new era, indeed conventional pla...
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ژورنال
عنوان ژورنال: CYTOLOGIA
سال: 2016
ISSN: 0011-4545,1348-7019
DOI: 10.1508/cytologia.81.359